This study focused on the development of an advanced municipal wastewater treatment process using a membrane-coupled anaerobic organic acid fermentor (MAOF) to remove dissolved organic matter from coagulated sludge. Intermittent ozone bubbling was effective in preventing increases in permeation resistance that were caused by particle accumulation on the membrane surface and in maintaining a high permeation flux. With regard to taxonomy, approximately 20 isolates were identified in the MAOF and are believed to represent the principal cell types in the fermentor. MAOF, coupled with intermittent ozone bubbling, is an effective system that can be implemented to recover organic matter that is used in biological denitrification or other basic materials.
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References
1.
AkohC., LeeG.C., ShawJ.F.2004. Protein engineering and applications of Candida rugosa lipase isoforms. Lipids, 39:513.
2.
American Public Health Association (APHA), American Water Works Association, and Water Environment Federation. 1998. Standard Methods for the Examination of Water and Wastewater, 20thClesceilL.S., GreenbergA.E., EatonA.E.Washington, DC: APHA.
3.
BérubéP.R., LeiE.2006. The effect of hydrodynamic conditions and system configurations on the permeate flux in a submerged hollow fiber membrane system. J. Membrane Sci., 271:29.
4.
BorjaR., BanksC.J.1994. Kinetic study of anaerobic digestion of fruit-processing wastewater in immobilized-cell bioreactors. Biotechnol. Appl. Biochem., 20:79.
5.
ChangI.S., Le ClechP., JeffersonB., JuddS.2002. Membrane fouling in membrane bioreactor for wastewater treatment. J. Environ. Eng-ASCE., 128:1018.
6.
ChangI.S., LeeC.H., AhnK.H.1999. Membrane filtration characteristics in membrane-coupled activated sludge system: The effect of floc stricture on membrane fouling. Separ. Sci. Technol., 34:1743.
7.
ChenK.C., LinY.F., HoungJ.Y.1997. Performance of a continuous stirred tank reactor with immobilized denitrifiers and methanogens. Water Environ. Res., 69:233.
8.
ChenM.1987. Difference in sporogenous bacterial populations in thermophilic (55°C) and mesophilic (35°C) anaerobic sewage digestion. Appl. Environ. Microbiol., 53:2414.
9.
ChooK.H., LeeC.H.1996. Effect of anaerobic digestion broth composition on membrane permeability. Water Sci. Technol., 34:173.
10.
CoxM., GerritseG., DankmeyerL., QuaxW.J.2001. Characterization of the promoter and upstream activating sequence from the Pseudomonas alcaligenes lipase gene. J. Biotechnol., 86:9.
11.
DartoisV., CoppéeJ.Y., ColsonC., BaulardA.1994. Genetic analysis and overexpression of lipolytic activity in Bacillus subtilis. Appl. Environ. Microbiol., 60:1670.
12.
De HaastJ., BritzT.J.1986. Characterization of aerobic and facultative anaerobic bacteria from the liquid phase of an anaerobic fixed-bed digester treating a cheese whey substrate. Microb. Ecol., 12:173.
13.
DemirelB., YenigunO.2002. Two-phase anaerobic digestion processes: A review. J. Chem. Technol. Biotechnol., 77:743.
14.
DreywoodR.1946. Qualitative test for carbohydrate material. Ind. Eng. Chem. Anal. Ed., 18:499.
15.
EspeelM., BriereN., CraemerD., JauniauxE., RoelsF.1993. Catalase-negative peroxisomes in human embryonic liver. Cell Tissue Res., 272:89.
16.
Essia NgangJ.J., WolniewiczE., LetourneauF., VillaP.1992. Stimulation of lactobacilli during alcoholic fermentation: Action of sucrose hydrolysis by yeast. Biotechnol. Lett., 14:741.
17.
GabelichC.J., YunT.I., CoffeyB.M., SuffetI.H.2002. Effects of aluminum sulfate and ferric chloride coagulant residuals on polyamide membrane performance. Desalination, 150:15.
18.
GuyotJ.P., RamirezF., OllivierB.1994. Synergistic degradation of acetamide by methanogens and an aerobic gram-positive rod. Appl. Microbiol. Biot., 42:452.
19.
HaverkampJ., MeuzelaarH.L.C., BeuveryE.C., BoonekampP.M., TiesjemaR.H.1980. Characterization of Neisseria meningitidis capsular polysaccharides containing sialic acid by pyrolysis mass spectrometry. Anal. Biochem., 104:407.
20.
JohnG.H., NoelR.K., PeterH.A.S., JamesT.S., StanleyT.W.1994. Bergey's Manual of Determinative and Systematic Bacteriology, 9th. Philadelphia, PA: Lippincott Williams & Wilkins.
21.
JouD., LlebotJ.E.1990. Introduction to the Thermodynamics of Biological Processes. Englewood Cliffs, NJ: Prentice Hall.
22.
KangI.J., YoonS.H., LeeC.H.2002. Comparison of the filtration characteristics of organic and inorganic membranes in a membrane-coupled anaerobic bioreactor. Water Res., 36:1803.
23.
KiriyamaK, AdachiT., TanakaY.1993. Field test of methane fermentation incorporating with membrane module for sewage sludge. J. Jpn. Sewage Works Ass., 16:127.
24.
LakshmideviR., MuthukumarK.2009. Enzymatic saccharification and fermentation of paper and pulp industry effluent for biohydrogen production. Int. J. Hydrogen Energ., 35:3389.
25.
Le ClechP., ChenV., FaneT.A.G.2006. Fouling in membrane bioreactors used in wastewater treatment. J. Membrane Sci., 284:17.
26.
LeeS.M., JungJ.Y., ChungY.C.2001. Novel method for enhancing permeate flux of submerged membrane system in two-phase anaerobic reactor. Water Res., 35:471.
27.
LettingaG, de ZeeuwW., OuborgE.1981. Anaerobic treatment of wastes containing methanol and higher alcohols. Water Res., 15:171.
28.
LiaoB.Q., KraemerJ., BagleyD.2006. Anaerobic membrane bioreactors: Application and research directions. Crit. Rev. Env. Sci. Tec., 36:489.
29.
LowryO.H., RosebroughN.J., FarrA.L., RandallR.J.1951. Protein measurement with Folin reagent. J. Biol. Chem., 193:265.
30.
MiyataT.1989. Study on high rate VFAs fermentation with municipal sewage coagulated sludge[Master's thesis]Department of Sanitary Engineering, Kyoto University: Japan.
31.
NakamuraY., SawadamT, MoritaY., TamiyaE.2002. Isolation of a psychrotrophic bacterium from the organic residue of a water tank keeping rainbow trout and antibacterial effect of violet pigment produced from the strain. Biochem. Eng. J., 12:79.
32.
NelL.H., BritzT.J., LateganP.M.1985. The effect of trace elements on the performance efficiency of an anaerobic fixed film reactor treating a petrochemical effluent. Water SA, 11:107.
33.
NoethC., BritzT.J., JoubertW.A.1988. The isolation and characterization of the aerobic endospore-forming bacteria present in the liquid phase of an anaerobic fixed-bed digester, while treating a petrochemical effluent. Microb. Ecol., 16:233.
34.
NtaikouI., KoutrosE., KornarosM.2009. Valorisation of wastepaper using the fibrolytic/hydrogen producing bacterium Ruminococcus albus. Bioresource. Technol., 100:5928.
35.
PaggaU., BeimbornD.B.1983. Anaerobic biodegradation test for organic compounds. Chemosphere, 27:1499.
36.
PitcherD.G., SaundersN.A., OwenR.G.1989. Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett. Appl. Microbiol., 8:151.
37.
RossB., StrohwaldH.1994. Membranes add edge to old technology. Water Qual. Int., 4:221.
SharmaV.K., HobsonP.N.1990. Isolation and characterization of a new Sarcina-like cellulolytic bacterium from an anaerobic, mesophilic, cattle-waste digester. Biol. Waste, 33:81.
40.
ShiY., ZhaoX., CaoP., HuY., ZhangL., JiaY., LuZ.2009. Hydrogen bio-production through anaerobic microorganism fermentation using kitchen wastes as substrate. Biotechnol. Lett., 31:1327.
41.
SomiyaI., KimD.H, TachikiY.1994. Evaluation of economy and treatment efficiency in advanced sewage treatment. J. Jpn. Sewage Works Ass., 31:32.
42.
ThompsonJ.D., HigginsD.G., GibsonT.J.1994. CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties, and weight matrix choice. Nucleic Acids Res., 22:4673.
43.
ToerienD.F.1967. Direct-isolation studies on the aerobic and facultative anaerobic bacterial flora of anaerobic digesters receiving raw sewage sludge. Water Res., 1:55.
44.
ToerienD.F., HattinghW.H.J.1969. Anaerobic digestion. I. The microbiology of anaerobic digestion. Water Res., 3:385.
45.
WiesnerM.R., LaînéJ.M.1996. Coagulation and membrane separation. MallevialleJ., OdendaalP., WiesnerM.R.Membrane Processes in Water Treatment. New York: McGraw-Hill.
46.
WilhelmS., TommassenJ., JaegerK.E.1999. A novel lipolytic enzyme located in the outer membrane of Pseudomonas aeruginosa. J. Bacteriol., 181:6977.
47.
WuF., ZhouS.2011. Continuous pro-hydrogen by anaerobic fermentation of municipal sludges. Int. J. Green Energy., 8:130.
48.
YanagiC., SatoM., TakaharaY.1994. Treatment of wheat starch wastewater by membrane combined two phase methane fermentation system. Desalination, 98:161.
49.
YeF.X., ShenD.S., FengX.S.2004. Anaerobic granule development for removal of pentachlorophenol in an upflow anaerobic sludge blanket (UASB) reactor. Process Biochem., 39:1249.
50.
ZeikusJ.G.1977. The biology of methanogenic bacteria. Bacteriol. Rev., 41:514.
