In this study, we induced a number of mutants of S. mutans differing in their abilities to synthesize glucan and produce acid. When tested for cariogenicity in gnotobiotic rats, "high pH" mutants caused less caries, but could not supplant a cariogenic parent strain.
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References
1.
Gibbons, R.J. and Van Houte , J.: Dental Caries, Ann Rev Med26:121-136, 1975.
2.
Fitzgerald, R.J.: Dental Caries Research in Gnotobiotic Animals , Caries Res2 :139-146,1968.
3.
Edwardsson, S.: Characteristics of Caries-inducing Human Streptococci Resembling Streptococcus mutans, Arch Oral Biol13: 637-646, 1968.
4.
Perch, B.; Kjems, E.; and Ravn, T.: Biochemical and Serological Properties of Streptococcus mutans from Various Human and Animal Sources, Acta Path Microbiol82(B):357-370, 1974.
5.
Shklair, 1. and Keene, A.: A Biochemical Scheme for the Separation of the Five Varieties of Streptococcus mutans, Arch Oral Biol19:1079-1081,1974.
6.
Bratthall, D. : Demonstration of Five Serological Groups of Streptococcal Strains Resembling Streptococcus mutans, Odontol Revy21:143-152, 1970.
7.
Michalek, S.M. ; Shiota, T.; Ikeda , T.; Navia, J.M.; and McGhee , R.: Virulence of Streptococcus mutans: Biochemical and Pathogenic Characteristics of Mutant Isolates, Proc Soc Exp Biol Med150:498-502, 1975.
8.
Kuramitsu, H.K. : Properties of a Mutant of Streptococcus mutans Altered in Glucosyl-transferase Activity, Infect Immun13 :345-353, 1976.
9.
Niven, C.F.; Smiley, K.L.;and Sherman, J.M.: The Hydrolysis of Arginines by Streptococci, J Bacteriol43:651, 1942.
10.
McCabe, R.; Keyes, P.; and Howell, A., Jr.: An in vitro Method for Assessing the Plaque-forming Ability of Oral Bacteria, Arch Oral Biol12:1653-1656, 1967.
11.
Rantz, L.A. and Randall, E.: Use of Autoclaved Extracts of Hemolytic Streptococci for Serological Grouping, Standford Med Bull13:290-291, 1955.
12.
Bright, J.S. ; Rosen, S.; and Chorpenn-Ing , F.W.: Survey of the Seven Serological Types of Streptococcus mutans in Six-year-old Children , J Dent Res56:1421, 1977.
13.
Rosen, S.: A Simple and Accurate Method for Determining Mean pH, Arch Oral Biol8:799, 1963.
14.
Lamberts, B.L. ;Meyer, T.S.;and Simon-Son L.G.: Investigation of Possible Solvents for Extracellular Polysaccharides from Streptococcus mutans and Streptococcus sanguis, J Dent Res54:857-866, 1975.
15.
Dahlqvist, A. ; Krasse, B.; Olsson , I.; and Gardell, S.: Extracellular Poly
16.
saccharides Formed by Caries-inducing Streptococci, Helv Odont Acta11:15-21, 1967.
17.
Dubois, M.: GILLES, K.A.; HAMILTON, J.K.; REBERS, P.A.; and SMITH, F.: Colorimetric Method for Determination of Sugars and Related Substances, Anal Chem28:350, 1956.
18.
Lowry, O.H. ; Rosebrough, N.J.; Farr, A.L.; and Randall, R.J.: Protein Measurement with the Folin Phenol Reagent, J Biol Chem193:265-275, 1951.
19.
Keyes, P.H. : Dental Caries in the Molar Teeth of Rats. II. A Method for Diagnosing and Scoring Several Types of Lesions Simultaneously , J Dent Res37:1077-1099, 1958 .
20.
Bratthall, D. and Kohler, B.: Streptococcus mutans Serotypes: Some Aspects of Their Identification, Distribution, Antigenic Shifts, and Relationship to Caries, J Dent Res55(C):15-21, 1976.
21.
Freedman, M.L. and Tanzer, J.M.: Dissociation of Plaque Formation from Glucan-induced Agglutination in Mutants of Streptococcus mutans, Infect Immun10: 189-196,1974.
22.
Shinefield, H.R.; Ribble, J.C.; Boris , M.: Bacterial Interference Between Strains of Staphylococcus aureus, 1960-1979, Am J Diseases Children121:148-152,1971.
