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Abstract

The modification of photoelectrical property of dried purple membrane (PM) film by acid treatment and deionization method is reported. By acid treatment and deionization, blue and acid purple form of bacteriorhodopsin, which have been obtained only in suspension, are able to be obtained in dried PM films. Using these films, the photoelectrical responses for blue and acid purple form of bacteriorhodopsin in dried films are measured. The disappearance of the normal photocur rent and appearance of a photocurrent with an opposite polarity is observed for blue and acid purple form of dried PM. The photoelectrical property of bR in dried PM is able to be modified by acid treatment and deionization. These treatments are able to control the polarity of bacteriorhodopsin photoresponse without change of the orientation of the bacteriorhodopsin molecules.

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References

Birge, R.R. 1990. "Photophysics and Molecular Electronic Applications of the Rhodopsins", Annu. Rev. Phys. Chem. , 41:683-733.

Drachev, L.A. , A.D. Kaulen , L.V. Khitrina and V.P. Skulachev. 1981. "Fast Stages of Photoelectric Processes in Biological Membranes", Eur. J. Biochem. , 117:461-470.

Fisher, U. and D. Oesterhelt. 1979. "Chromophore Equilibria in Bacteriorhodopsin" , Biophys. J. , 28:211-230.

Haronian, D. and A. Lewis. 1991. "Elements of a Unique Bacteriorhodopsin Neural Network Architecture", App. Opt. , 30(5):597-608.

Hong, F. T., ed. 1991. Molecular Electronics. New York: Plenum Press.

Keszthelyi, L. 1988. "Primary Charge Motions and Light-Energy Transduction in Bacteriorhodopsin", Biophysical Chemistry , 29:127-136.

Keszthelyi, L. and P. Ormos. 1989. "Protein Electric Response Signals from Dielectrically Polarized Systems", J. Membrane Biol. , 109:193-200.

Kimura, Y. , A. Ikegami and W. Stoeckenius. 1984. "Salt and pHDependence Changes of the Purple Membrane Absorption Spectrum", Photochemistry and Photobiology, 40(5):641-646.

Liu, S.Y. 1990. "Light-Induced Currents from Oriented Purple Membrane" , Biophysical J., 57:943-950.

10.

McIntosh, A.R. and F. Boucher. 1991. "On the Action Spectrum of the Photoelectric Transients of Bacteriorhodopsin in Solid State Film", Biochim. Biophys. Acta., 1056:149-159.

11.

Miyasaka, T. , K. Koyama and I. Itoh. 1992. "Quantum Conversion and Image Detection by a Bacteriorhodopsin-Based Artificial Photoreceptor", Science, 255:342-344.

12.

Mowery, P.C. , R. Lozier , Q. Chae , Y.W. Tseng , M. Taylor and W. Stoecknius. 1979. "Effect of Acid pH on the Absorption Spectra and Photoreactions of Bacteriorhodopsin", Biochemistry , 18(19):4100-4107.

13.

Ohtani, H. , T. Kobayashi and J. Iwai. 1986. "Picosecond and Nanosecond Spectroscopies of the Photochemical Cycles of Acidified Bacteriorhodopsin", Biochemistry, 25:3356-3363.

14.

Okajima, T.L. and F.T. Hong. 1986. "Kinetic Analysis of Displacement Photocurrents Excited in Two Types of Bacteriorhodopsin Model Membrane", Biophys. J., 50:901-912.

15.

Takei, H. , A. Lewis , Z. Chen and I. Nebenzahl. 1991. "Implementing Receptive Fields with Excitatory and Inhibitory Optoelectrical Responses of Bacteriorhodopsin Film", Appl. Opt. , 30(4):500-509.

16.

Varo, G. 1981. "Dried Oriented Purple Membrane Samples", Acta. Biol. Acad. Sci. Hung. , 32(3-4):301-310.

Photoelectrical Responses of Dried Purple Membrane Film in Blue Form and Acid Purple Form

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