Cells-to-C T Kits: Next Generation Gene Expression Analysis Workflows that Eliminate Sample Purification

Abstract

Real-time reverse transcription PCR analysis of RNA expression in normal and diseased states is a fundamental technique used in research and screening workflows. However, traditional purification of RNA samples often represents a significant workflow bottleneck, which increases the time and automation complexity required for analysis. We describe the Cells-to-C_T technology, a workflow that was designed in conjunction with real-time analysis to completely eliminate the need for traditional purification while maintaining performance relative to purified RNA. High-density sample preparation is accomplished in less than 8 min and is highly amenable to automation. We demonstrate that results are equivalent to RNA purified using traditional methods over a broad range of sample input while offering advantages when dealing with automated liquid handlers.