Natural dye was extracted from Cinnamomum camphora (L.) presl fruit and purified by aqueous two-phase extraction and the macro porous resin adsorption method. The extracted dye was characterized by Fourier transform infrared spectroscopy and liquid chromatography/mass spectrometry. The experimental results showed that the extraction process could be optimized by using a 1:1 mass ratio of water and ethanol as a solvent with the addition of 5 wt% Tween-80 for extracting at 50℃ for 2 h. The extracted dye was applied for the dyeing of wool fabrics, and the optimal dyeing conditions were found to be at 100℃ for 60 min by using 2.5 wt% of antimony potassium tartrate as a mordant. The kinetic study showed that the adsorption of the extracted dyes toward wool fabrics was fitted with the pseudo-second-order model. The dyed wool fabric exhibited excellent color fastness to washing, rubbing and light.