The aim of the present study was to investigate hyaluronic acid (HA) concentrations in vocal folds among patients with Reinke’s edema.
Study Design
Prospective and experimental study.
Setting
Single tertiary center.
Methods
An HA binding protein isolated from bovine nasal cartilage was used to identify and isolate the HA from samples. Plates coated with biotin-conjugated binding protein and streptavidin-europium conjugate were sequentially incubated with 18 Reinke’s edema samples and 11 female vocal fold cover samples from cadavers (the superficial layer of the lamina propria; control group). After the release of europium from streptavidin in enhancement solution, final fluorescence was measured in a fluorometer.
Results
The mean HA concentration in Reinke’s edema vocal folds was significantly higher than that in the control vocal folds (9.2 × 103 vs 0.9 × 103µg/g).
Conclusion
Vocal fold covers affected by Reinke’s edema present a higher concentration of HA than do vocal fold covers with no edema.
HiranoM. Structure of vocal fold in normal and disease states: anatomical and physical studies. ASHA Rep. 1981;11:11-30.
2.
HiranoM. Morphological structure of the vocal cord as a vibrator and its variations. Folia Phoniatr Logop. 1974;26(2):89-94.
3.
GraySD. Cellular physiology of the vocal folds. Otolaryngol Clin North Am. 2000;33(4):679-697.
4.
MeloECLemosMXimenes FilhoJSennesLUSaldivaPHTsujiDH. Distribution of collagen in the lamina propria of the human vocal fold. Laryngoscope. 2003;113(12):2187-2191.
5.
ChanRWGraySDTitzeIR. The importance of hyaluronic acid in vocal fold biomechanics. Otolaryngol Head Neck Surg. 2001;124(6):607-614.
6.
ButlerJEHammondTHGraySD. Gender-related differences of hyaluronic acid distribution in the human vocal fold. Laryngoscope. 2001;111(5):907-911
7.
GarciaACDCamposBMELopesCDRiveroJPerezPBLopesAD. Polyps, nodules and Reinke edema: an epidemiological and histopathological study. Acta Otolaryngol Esp. 1999;50(6):443-447.
8.
ChauHNDesaiKGeorgalasCHarriesM. Variability in nomenclature of benign laryngeal pathology based on video laryngoscopy with and without stroboscopy. Clin Otolaryngol. 2005;30(5):424-427.
9.
RoyNMerrillRMGraySDSmithEM. Voice disorders in the general population: prevalence, risk factors and occupational impact. Laryngoscope. 2005;115(11):1988-1995.
10.
GraySDHammondEHansonDF. Benign pathologic responses of the larynx. Ann Otol Rhinol Laryngol. 1995;104(1):13-18.
11.
TilmannBRudertHSchünkeMWernerJA. Morphological studies on the pathogenesis of Reinke’s edema. Eur Arch Oto Rhino Laryngol. 2011;252(8):469-474.
12.
SatoKHiranoMNakashimaT. Electron microscopic and immunohistochemical investigation of Reinke’s edema. Ann Otol Rhinol Laryngol. 1999;108(11):1068-1072.
13.
VolicSVKlapanISeiwerthSTbrahimpasicT. Extracellular matrix of Reinkes’s space in some pathological conditions. Acta Otolaryngol. 2004;124(4):505-508.
14.
BranskiRCSaltmanBSulicaL, et al. Cigarette smoke and reactive oxygen species metabolism: implications for the pathophysiology of Reinke’s edema. Laryngoscope. 2009;119(10):2014-2018.
15.
BranskiRCZhouHKrausDHSivasankarM. The effects of cigarette smoke condensate on vocal fold transepithelial resistance and inflammatory signaling in vocal fibroblasts. Laryngoscope. 2011;121(3):601-605.
16.
KravosAHocevar-BoltezarIGersakK. Serum levels of sex hormones in males with Reinke’s edema. Eur Arch Otorhinolaryngol. 2013;270(1):233-238.
17.
Díaz-FloresLGutiérrezRGarcíaMPÁlvarez-ArguellesHJuniorLDFCamposDL. CD34-positive fibroblasts in Reinke’s Edema. Laryngoscope. 2013;124(3):73-80(e).
18.
SakaeFAImamuraRSennesLUMauadTSaldivaPHTsujiDH. Disarrangement of collagen fibers in Reinke’s edema. Laryngoscope. 2008;118(8):1500-1503.
19.
SakaeFAImamuraRSennesLUTsujiDHMauadTSaldivaPH. Elastic fibers in Reinke’s edema. Ann Otol Rhinol Laryngol. 2010;119(9):609-614.
20.
OpheimLRHellmanUEngström-LaurentALaurentC. Hyaluronan in human vocal folds in smokers and nonsmokers—a histochemical study. J Voice. 2016;30(3):255-262.
21.
YonekawaH. A clinical study of Reinke’s edema. Auris Nasus Larynx. 1988;15(1):57-78.
22.
KornGPMartinsJRMParkSW, et al. Concentration of hyaluronic acid in human vocal folds in young and old subjects. Otolaryngol Head Neck Surg. 2011;145(6):981-986.
23.
TammiMIDayAJTurleyEA. Hyaluronan and homeostasis: a balancing act. J Biol Chem. 2001;277(7):4581-4584.
24.
HammondTHZhouRHammondEHPawlakAGraySD. The intermediate layer: a morphologic study of the elastin and hyaluronic acid constituents of normal human vocal folds. J Voice. 1997;11(1):59-66.
25.
LeblMDMartinsJRMNaderHBSimoesMJDe BiaseNG. Concentration and distribution of hyaluronic acid in human vocal folds. Laryngoscope. 2007;117(4):595-599.
26.
MartinsJRPasserottiCCMacielRMSampaioLODietrichCPNaderHB. Practical determination of hyaluronan by a new noncompetitive fluorescence-based assay on serum of normal and cirrhotic patients. Anal Biochem. 2003;319(1):65-72.
27.
TengbladA. Affinity chromatography on immobilized hyaluronate and its application to the isolation of hyaluronate binding proteins from cartilage. Biochem Biophys Acta. 1979;578:281-289.
28.
ZeitelsSMBuntingGWHillmanREVaughnT. Reinke’s edema: phonatory mechanisms and management strategies. Ann Otol Rhinol Laryngol. 1997;106(7):533-543
29.
CoyleSMWeinrichBDStempleJC. Shifts in relative prevalence of laryngeal pathology in a treatment-seeking population. J Voice. 2001;15(3):424-440.
30.
Van HoutteEVan LierdeKD’haeseleerEClaeysS. The prevalence of laryngeal pathology in a treatment-seeking population with dysphonia. Laryngoscope. 2010;120(2):306-312.
31.
IshiiKAkitaMYamashitaKHiroseH. Age-related development of the arrangement of connective tissue fibers in the lamina propria of the human vocal fold. Ann Otol Rhinol Laryngol. 2000;109(11):1055-1064.
32.
SatoKHiranoMNakashimaT. Fine structure of the human newborn and infant vocal fold mucosae. Ann Otol Rhinol Laryngol. 2001;110(5):417-424.
33.
SatoKUmenoHNakashimaTNonakaSHarabuchiY. Histopathologic investigations of the unphonated human child vocal fold mucosa. J Voice. 2012;26(1):37-43.
34.
HiranoMSatoKNakashimaT. Fibroblasts in human vocal fold mucosa. Acta Otolaryngol (Stockh). 1999;119(2):271-276.
35.
GraySDTitzeIRChanRHammondTH. Vocal fold proteoglycans and their influence on biomechanics. Laryngoscope. 1999;109(6):845-854.
36.
DouglasRSAfifyanNFHwangCJ, et al. Increased generation of fibrocytes in thyroid-associated ophthalmopathy. J Clin Endocrinol Metab. 2010;95(1):430-438.
37.
SatoKUmenoHNakashimaTNonakaSHarabuchiY. Expression and distribution of hyaluronic acid and CD44 in unphonated human vocal fold mucosa. Ann Otol Rhinol Laryngol. 2010;110(11):773-780.
38.
MarcotulioDMagliuloGPezoneT. Reinke’s edema and risk factors: clinical and histopathologic aspects. Am J Otolaryng. 2002;23(2):81-84.
39.
RemacleMDegolsJCDelosM. Exudative lesions of Reinkes’s space: an anatomopathological correlation. Acta Otorhinolaryngol Belg. 1996;50(4):253-264.
40.
LimSSauPCooperLMcPhadenAMackenzieK. The incidence of premalignant and malignant disease in Reinke’s edema. Otolaryngol Head Neck Surg. 2015;150(3):434-436.
