Restricted accessResearch articleFirst published online 1998-12
Spermidine Determines the Sensitivity to the Calmodulin Antagonist,Chlorpromazine,for the Circadian Conidiation Rhythm but Not for the Mycelial Growth in Neurospora crassa
The gene that suppresses the phenotype of the cpz-2mutation, which results in changing the sensitivity to chlorpromazine in relation to mycelial growth and circadian rhythms, was cloned in Neurospora crassa.This gene is not the cpz-2 gene itself but rather is identical to the spe-3gene that encodes spermidine synthase in Neurospora. The intracellular content of spermidine was lowered in the cpz-2strain compared to that of the wild-type strain. By integration of the spe-3 gene or by the addition of spermidine into culture medium, the temperature sensitivity of mycelial growth was lost and the conidiation rhythm became sensitive to chlorpromazine in the cpz-2 strain, as was observed in the wild-type strain, but the hypersensitivity of mycelial growth on chlorpromazine in the cpz-2strain was not affected. Therefore, it appears that spermidine determines only the sensitivity of the conidiation rhythm to chlorpromazine.
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