Gene Expression Underlying Teloblast Formation in Leech (Annelida: Hirudinae)

Abstract

Objective:

Species of glossiphoniid leech provide model systems for exploring fundamental questions in evolutionary and developmental biology. In this study, we took advantage of large embryonic cells and stereotypical cleavage patterns in the leech, Helobdella austinensis, to identify genes associated with the births of teloblasts and their lineage specifications.

Methods:

Using staged embryos and pools of dissected precursor cells and teloblasts, a systematic computational comparison of staged and cell type-specific transcriptomic data was employed to identify unique gene sets associated with mesodermal (M), neuroectodermal (N) and teloblast (M + N) cell formation.

Results and Conclusions:

Our predicted candidate genes comprised sets of nucleic acid binding factors as anticipated but displayed little similarity with differentiation factors in other metazoans (eg, mammals, cnidarians), suggesting that stem cell/lineage induction processes have diverged across the Animalia and may reflect fundamentally different modes of embryonic development.

Author Summary

Leeches are historically well known for bloodletting practices that were widespread throughout the 19th century and were also a classic preparation for neurobiologists during the same period. In modern times they have found medical applications in reconstructive surgeries, wound healing and more recently “hirudotherapy.” Lesser known are the contributions of leeches to embryology and evolution. Here we take advantage of unusually large embryos in the leech, Helobdella autinensis, to isolate homogeneous populations of teloblasts and their immediate precursor cells. By extracting RNA from these different cells, we were able to identify sets of genes that were unique to each cell type. Computational analyses of targeted genes suggest that many are associated with determining stem cell fate and lineage specification (eg, mesoderm, neuroectoderm). Interestingly, however, our predicted gene sets display only marginal similarity with comparable genes in other animals (eg, humans, Hydra), implying that early developmental processes associated with stem cell formation have diverged considerably over the course of animal evolution.