The evolution from first-generation through third-generation glucose sensors has witnessed the appearance of a number of very diverse oxidoreductases, which vary tremendously in terms of origin, structure, substrate specificity, cofactor used as primary electron acceptor, and acceptable final electron acceptor. This article summarizes our present knowledge of redox enzymes currently utilized in commercially available glucose monitoring systems to promote a fuller appreciation of enzymatic properties and principles employed in blood glucose monitoring to help avoid potential errors.
ClarkLCJrLyonsC. Electrode systems for continuous monitoring in cardiovascular surgery. Ann N Y Acad Sci.1962;102: 29–45.
2.
KeilinDHartreeEF. The use of glucose oxidase for the determination of glucose in biological material and for the study of glucose-producing systems by manometric methods. Biochem J.1948;42 (2): 230–8.
3.
RomanosMAScorerCAClareJJ. Foreign gene expression in yeast: A review. Yeast.1992;8 (6): 423–88.
4.
WittSSinghMKaliszHM. Structural and kinetic properties of nonglycosylated recombinant Penicillium amagasakiense glucose oxidase expressed in Escherichia coli. Appl Environ Microbiol.1998;64 (4): 1405–11.
5.
KleppeK. The effect of hydrogen peroxide on glucose oxidase from Aspergillus niger. Biochemistry.1966;5 (1): 139–43.
6.
BaoJFurumotoKFukunagaKNakaoK. A kinetic study on air oxidation of glucose catalyzed by immobilized glucose oxidase for production of calcium gluconate. Biochem Eng J.2001;8: 91–102.
7.
MirónJGonzálezMPPastranaLMuradoMA. Diauxic production of glucose oxidase by Aspergillus niger in submerged culture–a dynamic model. Enz Microb Technol.2002;31: 615–20.
8.
VijayakumarARCsóregiEHellerAGortonL. Alcohol biosensors based on coupled oxidase-peroxidase systems. Anal Chim Acta.1996;327 (3): 223–34.
9.
KatzEWillnerI. Biomolecular architecture: Routes to biosensors, bioelectronics and biofuel cells. In: Proceedings of the First International Symposium on Macro- and Supramolecular Architectures and Materials (MAM-01): Biological and Synthetic Systems, Kwangju, South Korea, 10–14 April, 2001, 78–81.
10.
AlbersMWLekkalaJOJeukenLCantersGWTurnerAP. Design of novel molecular wires for realizing long distance electron transfer. Bioelectrochem Bioeng.1997;42: 25–33.
11.
TsaiYCLiSCChenJM. Cast thin film biosensor design based on a Nafion backbone, a multiwalled carbon nanotube conduit, and a glucose oxidase function. Langmuir.2005;21 (8): 3653–8.
CourjeanOGaoFManoN. Deglycosylation of glucose oxidase for direct and efficient glucose electrooxidation on a glassy carbon electrode. Angew Chem Int Ed Engl.2009;48 (32): 5897–9.
14.
WohlfahrtGWittSHendleJSchomburgDKaliszHMHechtHJ. 1.8 and 1.9 A resolution structures of the Penicillium amagasakiense and Aspergillus niger glucose oxidases as a basis for modelling substrate complexes. Acta Crystallogr D Biol Crystallogr.1999;55 (Pt 5): 969–77.
15.
SouthallSMDoelJJRichardsonDJOubrieA. Soluble aldose sugar dehydrogenase from Escherichia coli: A highly exposed active site conferring broad substrate specificity. J Biol Chem.2006;281 (41): 30650–9.
16.
IgarashiSOhteraTYoshidaHWitartoABSodeK. Construction and characterization of mutant water-soluble PQQ glucose dehydrogenases with altered K(m) values–site-directed mutagenesis studies on the putative active site. Biochem Biophys Res Commun.1999;264 (3): 820–4.
17.
OubrieARozeboomHJKalkKHOlsthoornAJDuineJADijkstraBW. Structure and mechanism of soluble quinoprotein glucose dehydrogenase. EMBO J.1999;18 (19): 5187–94.
SodeKIgarashiSMorimotoAYoshidaH. Construction of engineered water-soluble PQQ glucose dehydrogenase with improved substrate specificity. Biocatal Biotransform.2002;20: 405–12.
20.
IgarashiSHirokawaTSodeK. Engineering PQQ glucose dehydrogenase with improved substrate specificity. Site-directed mutagenesis studies on the active center of PQQ glucose dehydrogenase. Biomol Eng.2004;21 (2): 81–9.
21.
HamamatsuNSuzumuraANomiyaYSatoMAitaTNakajimaMHusimiYShibanakaY. Modified substrate specificity of pyrroloquinoline quinone glucose dehydrogenase by biased mutation assembling with optimized amino acid substitution. Appl Microbiol Biotechnol.2006;73 (3): 607–17.
22.
SodeKOoteraTShirahaneMWitartoABIgarashiSYoshidaH. Increasing the thermal stability of the water-soluble pyrroloquinoline quinone glucose dehydrogenase by single amino acid replacement. Enzyme Microb Technol.2000;26 (7): 491–6.
23.
IgarashiSSodeK. Stabilization of quaternary structure of water-soluble quinoprotein glucose dehydrogenase. Mol Biotechnol.2003;24 (2): 97–104.
24.
TanakaSIgarashiSFerriSSodeK. Increasing stability of water-soluble PQQ glucose dehydrogenase by increasing hydrophobic interaction at dimeric interface. BMC Biochem.2005;6: 1.
25.
YoshidaHArakiNTomisakaASodeK. Secretion of water soluble pyrroloquinoline quinone glucose dehydrogenase by recombinant Pichia pastoris. Enzyme Microb Technol.2002;30 (3): 312–8.
26.
KohHIgarashiSSodeK. Surface charge engineering of PQQ glucose dehydrogenase for downstream processing. Biotechnol Lett.2003;25 (20): 1695–701.
27.
GiardinaPde BiasiMGde RosaMGambacortaABuonocoreV. Glucose dehydrogenase from the thermoacidophilic archaebacterium Sulfolobus solfataricus. Biochem J.1986;239 (3): 517–22.
28.
SharmaVKumarVArchanaGKumarGN. Substrate specificity of glucose dehydrogenase (GDH) of Enterobacter asburiae PSI3 and rock phosphate solubilization with GDH substrates as C sources. Can J Microbiol.2005;51 (6): 477–82.
29.
PaulyHEPfleidererG. Conformational and functional aspects of the reversible dissociation and denaturation of glucose dehydrogenase. Biochemistry.1977;16 (21): 4599–604.
30.
YamaokaHYamashitaYFerriSSodeK. Site directed mutagenesis studies of FAD-dependent glucose dehydrogenase catalytic subunit of Burkholderia cepacia. Biotechnol Lett.2008;30 (11): 1967–72.
31.
TsujimuraSKojimaSKanoKIkedaTSatoMSanadaHOmuraH. Novel FAD-dependent glucose dehydrogenase for a dioxygen-insensitive glucose biosensor. Biosci Biotechnol Biochem.2006;70 (3): 654–9.
32.
OguraYNagahisaM. Untersuchungen über die Atmung und die Dehydrasesysteme von Aspergillus oryzae. Bot Mag Tokyo.1937;51 (606): 597–612.
33.
BakTG. Studies on glucose dehydrogenase of Aspergillus oryzae. II. Purification and physical and chemical properties. Biochim Biophys Acta.1967;139 (2): 277–93.
MoriKNakajimaMKojimaKMurakamiKFerriSSodeK. Screening of Aspergillus-derived FAD: -glucose dehydrogenases from fungal genome database. Biotechnol Lett. July 12, 2011. Epub ahead of print.
36.
JanssenWHarffGCaersMSchellekensA. Positive interference of icodextrin metabolites in some enzymatic glucose methods. Clin Chem.1998;44 (11): 2379–80.
