The volatile components isolated from leaves of Vepris madagascarica (Baillon) H. Perier (Rutaceae), an endemic species of north-eastern, sub-humid forests of Madagascar, were investigated by GC (Retention Indices), GC-MS and 13C NMR spectroscopy. Oil samples obtained on laboratory and industrial scales exhibited similar composition, dominated by phenylpropanoids. (E)-anethole (78.2% and 78.6%) was the major component followed by estragole (15.6% and 15.4%). In addition, trunk bark oil also contained (E)-anethole as its major component (84.6%), as well as various sesquiterpenes in low contents.
BilletD., Favre-BonvinJ. (1973) Constituants de l'huile essentielle de Vepris madagascarica. Phytochemistry, 12, 1194.
3.
PoitouF., MasottiV., VianoJ., GaydouE.M., AndriamahavoN.R., MamitianaA., RabemanantsoaA., RazanamahefaB.V., AndriantsiferanaM. (1995) Chemical composition of Vepris elliotii essential oil. Journal of Essential Oil Research, 7, 447–449.
4.
SurburgH., PantenJ. (2005) Common fragrance and flavor materials. Preparation, properties and uses. 5th completely revised and enlarged edition. Wiley-VCH Verlag GmbH & Co. KGaA.
5.
OttavioliJ., CasanovaJ., BighelliA. (2010) Composition and chemical variability of the leaf oil from Corsican Juniperus thurifera integrated analysis by GC(RI), GC-MS and 13C NMR. Natural Product Communications, 5, 1991–1994.
6.
(a) Van Den DoolH., KratzP.D. (1963) A generalization of the retention index system including linear temperature programmed gas–liquid partition chromatography. Journal of Chromatography, 11, 463–471;(b) Terpenoids Library Website. http://massfinder.com/wiki/Terpenoids_Library.
7.
(a) US National Institute of Standards and Technology PC Version 1.7 of the NIST/EPA/NIH Mass Spectra Library, Norwalk, CT (1999); (b) Adams RP. (2007) Identification of essential oil components by gas chromatography/mass spectroscopy. Allured Publishing Corporation, Carol Stream, Ill.
8.
(a) RezziS., BighelliA., CastolaV., CasanovaJ. (2002) Direct identification and quantitative determination of acidic and neutral diterpenes using 13C-NMR spectroscopy. Application to the analysis of oleoresin of Pinus nigra. Applied Spectroscopy, 56, 312–317; (b) Tomi F, Casanova J. (2006) 13C-NMR as a tool for identification of individual components of essential oils from Labiatae. A review. Acta Horticulturae, 723, 185–192.
