The Crambe tataria glucosinolate/myrosinase system in seeds and leaves of in vivo and in vitro regenerated plantlets, and two callus cell lines was investigated. It was demonstrated that in all the extracts glucosinolates were present and the myrosinase system was operative. There appears to be no discrimination between the glucosinolates used as substrates, but the hydrolysis rates were different regardless of the nature of the side chain. This is one of the first studies demonstrating that undifferentiated cells are able to synthetize glucosinolates and have an operating myrosinase system.
PoldiniL. (1973) Ad floram italicam notulae taxonomicae et geobotanicae “Crambe tataria“ Sebeók. Webbia., 28, 31–36.
2.
NimisP.L., FondaG. (1997) Phytogeography of parasteppic vegetation in the Friulan Plain (NE Italy). Plant Ecology, 132, 15–28.
3.
HechtS.S. (2000) Inhibition of carcinogenesis by isothiocyanates. Drug Metabolism Reviews, 32, 395–411.
4.
MichelsK.B., GiovannucciE., JoshipuraK.J., RosnerB.A., StampferM.J., FuchsC.S., ColditzG.A., SperizerF.E., WillettW.C. (2000) Prospective study of fruit and vegetable consumption and incidence of colon and rectal cancers. Journal of the National Cancer Institute, 92, 1740–1752.
5.
PappaG., LichtenbergM., IoriR., BarillariJ., BartschH. (2006) Comparison of growth inhibition profiles and mechanisms of apoptosis induction in human colon cancer cell lines by isothiocyanates and indoles from Brassicaceae. Mutation Research, 599, 76–87.
6.
ScalbertA., Andres-LacuevaC., AritaM., KroonP., ManachC., Urpi-SardaM., WishartD. (2011) Databases on food phytochemicals and their health-promoting effects. Journal of Agricultural and Food Chemistry, 59, 4331–4348.
7.
RaskL., AndreassonE., EkbomB., ErikssonS., PontoppidanB., MeijerJ. (2000) Myrosinase: gene family evolution and herbivore defense in Brassicaceae. Plant Molecular Biology, 42, 93–113.
8.
IUCN (2006) 2006 IUCN red list of threatened species.www.iucnredlist.org. Accessed 04 May 2006.
9.
PiovanA., CassinaG., FilippiniR. (2011) Crambe tataria: actions for ex situ conservation. Biodiversity and Conservation, 20, 359–371.
10.
FavrettoD., PiovanA., FilippiniR., CaniatoR. (2001) Control of the production yields of vincristine and vinblastine in Catharanthus roseus from somatic embryogenesis. Semiquantitative determination by flow injection-electrospray ionization mass spectrometry. Rapid Communications in Mass Spectrometry, 15, 364–369.
11.
FilippiniR., CaniatoR., PiovanA., CappellettiE.M. (2003) Production of anthocyanins by Catharanthus roseus. Fitoterapia, 74, 62–67.
12.
PiovanA., CaniatoR., CappellettiE.M., FilippiniR. (2010) Organogenesis from shoot segments and via callus of endangered Kosteletzkya pentacarpos (L.) Ledeb. Plant Cell Tissue and Organ Cultures, 100, 309–315; (b) Piovan A, Cozza G, Caniato R, Moro S, Filippini R. (2010) A novel glucosyltransferase from Catharanthus roseus cell suspensions. Process Biochemistry, 45, 655–659; (c) Wielanek M, Urbanek H. (1999) Glucotropaeolin and myrosinase production in hairy root cultures of Tropaeolum majus. Plant Cell Tissue and Organ Cultures, 57, 39–45.
13.
AlvarezS., HeY., ChenS. (2008) Comparative investigations of the glucosinolate–myrosinase system in Arabidopsis suspension cells and hypocotyls. Plant Cell Physiology, 49, 324–333; (b) Bernardi R, Finiguerra MG, Rossi AA, Palmieri S. (2003) Isolation and biochemical characterization of a basic myrosinase from ripe Crambe abyssinica seeds, highly specific for epi-progoitrin. Journal of Agricultural and Food Chemistry, 51, 2737–2744; (c) Murashige T, Skoog F. (1962) A revised medium for rapid growth and bio assays with tobacco tissue culture. Physiologia Plantarum, 15, 473–497; (d) Palmieri S, Iori R, Leoni O. (1986) Myrosinase from Sinapis alba L.: a new method of purification for glucosinolate analyses. Journal of Agricultural and Food Chemistry, 34, 138–140.
