Abstract
HX0969w is a novel carboxylate ester prodrug of propofol. After intravenous administration of HX0969w, the compound is rapidly hydrolyzed to its active metabolite propofol. A liquid chromatography–tandem mass spectrometry method with electrospray ionization has been developed for determination of HX0969w in rabbit whole blood. Protein precipitation with methanol was used for sample preparation and 7-hydroxycoumarin served as internal standard. The standard curve ranged from 50.1 ng mL−1. to 15,030 ng mL−1. The lower limit of quantification for HX0969w was 50.1 ng mL−1. The intra- and inter-day accuracies were within ±10% and precisions were below 5.52%. The method was successfully applied to samples from a rabbit pharmacokinetic study.
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