Abstract
While the chemical information provided by electric dichroism spectroscopy can be useful for studying the conformation and electronic structures of molecules in solution, the dichroism signals produced at fields approaching the dielectric strength of organic solvents are extremely small and difficult to detect. A double-beam, laser-based instrument for electric dichroism measurements is described which allows detection of dichroism signals corresponding to changes in absorbance of less than 10−6 with only a 1.2-kV high-voltage source.
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References
1.
Kuhn
W.
Duhrkip
H.
, and
Martin
H.
, H. Z. Phys. Chem. B 45 , 121 (1939 ).
2.
Allen
F. S.
and
Van Holde
K. E.
, Biopolymers 10 , 865 (1971 ).
3.
Fredericq
E.
and
Houssier
C.
, Electric Dichroism and Electric Birefringence (Clarendon Press , Oxford , 1973 ).
4.
Yamaoka
K.
and
Charney
E. J.
, J. Amer. Chem. Soc. 94 , 8963 (1972 ).
5.
Crothers
D. M.
Dattagupta
N.
, and
Hogen
M.
, Nucleic Acid Geometry and Dynamics ,
Sarma
R. H.
, Ed. (Pergamon Press , New York , 1980 ), pp. 341 –349 .
6.
Tsuji
K.
and
Neumann
E.
, Biophys. Chem. 17 , 153 (1983 ).
7.
O'Konski
C. T.
Yoshioka
K.
, and
Orttung
W. G.
, J. Phys. Chem. 63 , 1558 (1959 ).
8.
Schachter
E. M.
Bendet
I. J.
, and
Lauffer
M. A.
, J. Mol. Biol. 22 , 165 (1966 ).
9.
Charney
E.
, Procedures in Nucleic Acid Research ,
Cantoni
G. L.
, Ed. (Harper & Row , New York , 1971 ), Vol. 2 , pp. 176 –204 .
10.
Davidsson
A.
and
Norden
D.
, Spectrosc. Lett. 10 , 447 (1977 ).
11.
Causley
G. C.
Scott
J. D.
, and
Russell
B. R.
, Rev. Sci. Instrum. 48 , 264 (1977 ).
12.
Rothman
L. D.
Crouch
S. R.
, and
Ingle
J. D.
, Anal. Chem. 47 , 1226 (1975 ).
13.
Currie
L. A.
, Anal. Chem. 40 , 586 (1968 ).
