This study presents in-depth scanning electron microscopic (SEM) and energy-dispersive X-ray spectroscopic (EDX) analyses of the surface contour of natural bamboo fibers from Phyllostachys rubromarginata species processed under various eco-friendly conditions. Both fresh and dry bamboo were used. Fresh bamboo provided easier and quicker processing. Two age groups, 6-months to 1-year-old and 2 to 4-year-old bamboo plants, were also studied for fiber output. Subjective visual observations suggested that the age of the bamboo affected fiber yield. The diameter of the bamboo fibers was 10-17 μm, which fits within standard spinning parameters. Analysis showed that the bamboo fibers were well-rounded along the longitudinal direction, unlike bamboo viscose typically found on the market. This article provides a detailed description of some successful processes for bamboo fiber production.