Association of Genetic Polymorphisms in the Paraoxonase 1 Gene with the Risk and Prognosis of Non–Small Cell Lung Cancer in Chinese Han Population

Abstract

Objective

Oxidative stress and free radicals have been indicated to be associated with increased risk of various cancers. Paraoxonase 1 (PON1) gene, encoding PON1 protein, plays an important role as an endogenous free-radical scavenging molecule. The aim of the present study was to determine whether genetic polymorphisms of the PON1 gene are associated with the risk and prognosis of non–small cell lung cancer (NSCLC).

Methods

A total of 356 patients with NSCLC and 184 healthy controls were enrolled in this study. Q192R and L55M polymorphisms of the PON1 gene were genotyped using polymerase chain reaction and restriction fragment length polymorphism method.

Results

As for the Q192R polymorphism, the patients with NSCLC showed higher frequencies of QR and RR genotypes than the normal controls. Unconditional logistic regression analysis revealed that QR and RR genotypes were significantly associated with the presence of NSCLC compared with the QQ genotype. No significant differences were found in the genotypes of the L55M polymorphism between the patients with NSCLC and the healthy controls. The QR and RR genotypes were significantly associated with TNM stage and nodal metastasis. Survival analysis indicated that the patients with NSCLC with the QR and RR genotypes had poorer disease-free survival than those with the QQ genotype (P = 0.011). Multivariable Cox regression analysis revealed that QR and RR genotypes were an independent factor for the prognosis of NSCLC (hazard ratio, 1.505; 95% confidence interval, 1.004–2.256; P = 0.048).

Conclusion

The Q192R polymorphism of the PON1 gene may be associated with the risk and prognosis of NSCLC in a Chinese Han population.